DEVELOPMENT OF A NEW ANIMAL FEED SUPPLEMENT FOR IMPROVING ANIMALS PERFORMANCE

Acronim: ZINCOPPYEAST

PROJECT NUMBER : NR. 94 ⁄ 2017

Project Coordonator : AVICOLA LUMINA SA

PROJECT MANAGER :Drd. Ing. VASILE BUNDUC, director AVICOLA LUMINA SA

Financed by  UEFISCDI – Program  PN III: Cooperare Europeană și Internațională

Subprogram name: Subprogram 3.5. Other European and international initiatives and programs  EUREKA Tradiţional, EUREKA-Cluster Project , Eurostars

Project value: Link to the page UEFISCDI: www.uefiscdi.gov.ro

Budget RO 333.333 EURO

SELF FUNDING RO: 212.360 EURO

Total : 545.693,00 EURO

Start date of the contract: 03/11/2017

Date of finalization of the contract: 03/11/2020

Project end date (including the reporting period for economic effects: 03/11/2023

Consortium component:

Project Coordonator: AVICOLA LUMINA SA . Reprezentant Legal  Drd.ing.Vasile Bunduc . Director Proiect  Drd.ing.Vasile Bunduc            

Partner 1 : PHARMACORP INNOVATION. Reprezentant Legal  Dr.ing.Diana BARBULESCU . Responsabil de proiect P1 Dr.ing.Diana BARBULESCU. 

Partner2 : AGSIRA SRL . Reprezentant Legal  Ing.NEGRILA  Radian  Nicolae . Responsabil de proiect P 2 Ing.NEGRILA  Radian
Nicolae.

Partner3 :  INSTITUTUL NATIONAL DE CERCETARE-DEZVOLTARE PENTRU BIOLOGIE SI NUTRITIE ANIMALA –
IBNA BALOTESTI . Reprezentant Legal  Prof. Dr. Horia GROSU. Responsabil de proiect P3 Dr. Daniela MARIN .

Partner 4: Szent István University, Dept. Appl. Chem., Faculty of Food Science (HUNGARY)–P4 . Represented by  János Tőzsér, Dr. Rector and  Ferenc Magyar Chancellor. Jókainé Dr. Zsuzsanna Szatura. 

Partner 5 : ADEXGO Kft. Represented by Tamás Tóth Dr. (Managing Director). Dr. Hajnalka Hingyi .                                                            

Contact : Ing. VASILE BUNDUC – tel:0040 241 251 050, email: vasile.bunduc@avicolalumina.ro

Abstract:

The main scientific and technical goal of this project is to obtain innovative products: an animal feed supplement for new feed recipes, mainly based on a blend between spent brewer yeast from the brewers industries with the wet yeast biomass enriched with microelements (organic zinc), polyphenols and vitamins and also a blend between spent brewing yeast with inorganic zinc. The comparative test will be made in vivo on the laying hens and the antioxidant activity will be evaluated.

Through these activities the ZINCOPPYEAST consortium will contribute to a better characterization of the new products and will provide the scientific knowledge concerning the effect of the new product on the general health of the laying hens and their capacity to improve the response to stress. The valorisation of spent brewing yeast will be very important for the environment as it was considered a waste. Also, in this project, we will determine the homogeneity, shelf life and ingredient stability of the zinc, vitamin and yeast-containing supplement in different laying hen premixes, concentrates and compound feeds.

During the production of poultry feed several feed technological procedures are carried out which involve the use of high temperature (pelletizing-crumbing, hygienization, expansion). The organoleptic (sensory) characteristics of eggs produced by hens. The innovative bio-ingredients as new feed supplement for new feed receipts will be patented.

Through the fundamental objectives, the project will increase multidisciplinary transnational technological cooperation between technological research centers from countries such as Romania and Hungary.

Project objective:

The main scientific and technical goal of this project is to obtain innovative products: an animal feed supplements for new feed receipts, mainly based on blended between spent brewer’s yeast from the brewers industries with the wet yeast biomass enriched with microelements (organic zinc),polyphenols, and vitamins and also blended between spent brewing yeast with inorganic zinc.The comparative test will be made in vivo on the laying hens and the antioxidant activity will be evaluated.

The main objectives of the project are:

– To obtain a new feed bio-ingredient, an yeast biomass enriched in organic zinc, polyphenols and valorization of spent brewing yeast;

This new product based on blended between spent brewer’s yeast from the brewers industries with the wet yeast biomass enriched in zinc organic, vitamin, polyphenols will drive to an innovative feed supplement for laying hens.

This will be comparative tested with other new feed supplement based on

dry spent brewing yeast with inorganic zinc in the new recipe for laying hens.

-To identify and characterize the in vivo effects of the new feed supplement on laying hen’s health.

The project will investigate the effect of the new combinations, new mixtures between spent brewing yeast and  wine or with yeast consortium (wine and beer yeast) wet biomass enriched with Zn and polyphenols, in order to produce an innovative feed receipt, a new feed supplement which will be tested on the laying hens with further commercialization on the market.

Estinated results:

By using the  mentioned technologies in project the consortium will develop two categories of products:

– bioingredient/bioingredients, which are considered innovative for the feed supplement industry

– a new feed recipes for the laying hen’s feed.

 

Work plan stages

WP I. Development and new design of bio ingredients and proposed  new feed receipes.Nutrition program.  Dissemination of information. Design and proiection  of new bioingrediente new feed recipes and nutrition program design.- Partial

WP II. Development and new design of bio ingredients and proposed  new feed receipes.Nutrition program.  Dissemination of information Design and proiection  of new bioingrediente new feed recipes and nutrition program design. – Completion of activity  and  Development of new bio ingredients based on zinc and polyphenols . Experimental evaluation of them in vivo, to be used as a feed additive for laying hens antioxidant-enriched in zinc and polyphenols.(partial)

WP III SCALE UP  at pilot level. Testing of new feed receipes at pilot level) (partial)

WP  IV  Testing of new feed receipes at pilot level and  scale up  at industrial level

WP V Economic effect

WP VI Economic effect

WP  VIII  Economic effect

Stage 1. Development and design of new bio ingredients, new feed recipes and designing the nutrition program -Partially- Design of the project web page. Dissemination of information. (3.11.2017-31.12.2017).

In stage 1 of the “ZINCOPPYEAST” project, more than 30 colonies of wine and beer yeast were isolated. The majority have been tested for the influence of the carbon source used both for the development of inoculum and for the fermentation medium over the biomass development.

– the influence of the zinc on the biomass development was also tested;

– the technical specification for the beer yeast was made regarding the protein content, and the total number of germs;

– the main objective of this step was to optimize the extraction methods of total polyphenols  from yeast residues and to characterize some yeast residues from the beer industry in terms of content in total polyphenols.

– also, new nutritional solutions that use the yeast in the feed of laying hens have been achieved and a nutrition program was proposed;

– the project’s web page was created.

 

 

 

 

 

 

 

Wine and beer yeast colonies

 

 

 

 

 

 

 

 

 

 

Isolated wine and beer yeast colonies

Activity I.1. PARTIALIsolation of wine and beer yeast strains in order to be used alone or in a consortium for obtaining new bioingredients based on yeast consortium biomass enriched in zinc, that will be used to valorise  through  mixture with the used beer yeast yielding a product based on organic zinc, polyphenols,  and  bioingredients based on yeast biomass rich in zinc –

Activity I.2 Elaboration of nutrition program- PARTIAL

Activity 1.3 Dissemination of information Project Website

STAGE II. Elaboration and design of new bioingredients, new feed recipes and nutrition program design – (FINALIZATION) and Development of new bioingredients based on biomass of zinc / organic zinc yeasts, polyphenols and their experimental evaluation, in vivo, to be used as a supplement with an antioxidant role in the feed laying hens, enriched in zinc and polyphenols. Results dissemination – article / presentation at a conference (Partial)

ACTIVITIES OF STAGE 2

Act 2.1 – Isolation of brewer’s yeast strains to obtain new bioingredients based on zinc and polyphenols enriched yeasts, to be used by blending with the spent beer yeast. COMPLETION

Act 2.2 – Elaboration of nutrition program – Completion

Act 2.3 – Selection of isolated wine and beer yeasts and identification of those selected for obtaining bioingredients based on zinc, polyphenols yeast biomass that uses spent beer yeast and the effect on performances, health status, the oxidative status and polyphenol content at hens. Selection of raw materials for new formulas for obtaining feed additives with the help of innovative bioingredients based on yeasts enriched in zinc and polyphenols. Screening bioproducts. -Setting the parameters that define the effectiveness of new recipes.

Act 2.4 – Optimizing processes and new feed recipes at pilot level and verifying through nutrition experiments the effectiveness of new optimal recipes used to produce eggs enriched with zinc,  polyphenols. Publication

Act 2.5 – Study of the effect of bio-ingredients on performances, health status on oxidative status and polyphenol content in laying hens – partially

The main objective of this step was to isolate and identify yeast strains by molecular sequencing in order to obtain new bio-ingredients based on zinc-enriched yeasts (P1) mixed with the spent brewing yeast from (P2) obtaining the product (ZINCOPPYEAST), tested by the Project Coordinator (CO) in the feed of laying hens and analyzed by partner (P3) in terms of polyphenol content and antioxidant activity.

Analyzes of total zinc, organic zinc and polyphenols were made by partner P4.

By scale-up by optimization, the zinc enriched yeast mixture with the yeast enriched with zinc with spent beer yeast was made obtaining a mg / kg yeast content of  Organic Zn> 65, a total of 85 zinc total mg / kg of yeast.

-ZINCOPPYEAST presents, after the mixture, a total of 250 mg / 100g total polyphenols (referred to gallic acid).

-The total zinc content of the ZINCOPPYEAST feed was 160 mg / kg of yeast Zn Total and> 140 mg / kg  Organic Zn yeast.

-The polyphenol content of the ZINCOPPYEAST feed was 170 mg / 100g

– total polyphenol analysis determined a significant increase of polyphenols in the batch (ZINCOPPYEAST)

– Optimized recipes for the feed of laying hens have been achieved by CO.

– Different premixes and concentrate recipes were made by partner P5.

 

 

 

 

 

 

 

 

Selected  yeasts for ZINCOPPYEAST’biongredient obtainment