Summary, stage 4
The main activities performed during this stage were as follows:
- SC Hofigal SA (Partner 2) produced two new feed additives (AFC 5 and AFC 6) enriched in lutein and zeaxanthin and in other nutrients, which were characterized in terms of feeding value by the Chemistry laboratory of Partner 1 (IBNA Balotesti). The chemical assays showed that:
- AFC 5 feed additive consists of: alfalfa meal (dried, ground), red ear corn (dried, ground), pumpkin pulp (dried, ground), marigold flowers (dried, ground), crowfoot (dried, ground). AFC 6 feed additive consists of: buckthorn fruits (dried, ground), pumpkin pulp (dried, ground), red ear corn (dried, ground), marigold flowers (dried, ground), spirulina -powder.
- The lutein and zeaxanthin in AFC 6 (1408.498 mg/kg) was almost double compared to AFC 5 (738.075 mg/kg).
- The feed additives AFC 5 and AFC 6, besides their rich content of carotenoids, also have a high content of essential nutrients for animals, such as proteins, glucydes, lipids, vitamins and minerals.
- Methionine, limiting amino acid for poultry, was I n higher concentration in AFC 6. The lysine and cystine concentrations were also higher in AFC 6
- The Pb and Cd levels, in both feed additives, were below the maximal limits allowed by the acting regulations (Order 74/2011 of ANSVSA transposing Regulation (EU) no. 574/2011)
- The antioxidant capacity of AFC 6 (15.27±0.91 mM Trolox equivalents/g) was two times higher than in AFC 5 (7.37±0.78 mM Trolox equivalents /g), mainly due to the presence of spirulina in AFC 6.
- CO and P1 developed several diet formulations for poultry, using the two new feed additives, which are to be tested, in small scale trials, during the next stage.
- The purpose is to enrich the egg yolk in lutein and zeaxanthin using AFC 5 and AFC 6. Besides the conventional formulation (C), we also developed formulations that include conventional ingredients (alfalfa and corn gluten) as sources of xanthophylls.
- During this stage we also conducted several dissemination activities. The disseminated papers were written by collectives of authors from all the project partners. In the XVI European Symposium on the Quality of Eggs and Egg Products (10-13 May 2015) Nantes, France we had two poster presentations:
- Evolution of lutein and zeaxanthin concentration in the yolk of layers fed lutein-enriched diets; Authors: Bunduc, V., Panaite, T. D., Varzaru, I., Cornescu, M. G., Panaite, C. V., Pop, M.
- Influence of botanic mixes high in carotenoids (lutein and zeaxanthin) used in layer diets on the quality parameters of the eggs; Authors: Panaite, T. D., Bunduc, V., Criste, R. D., Cornescu, M. G.
- 1 paper on the chemical characterization of the feed additives, prepared by P2 has been accepted for publication in Serbia (Food and Feed Research, Journal of the Institute of Food Technology in Novi Sad);